Knockout cell lines
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??Engineered and ready for you
?CRISPR-Cas9 knockout cell lines from Abcam provide you with reliable, off-the-shelf, single-gene knockouts so that you can confidently interrogate the relationship between genotype and phenotype without having to establish your own knockout cell line.
Reliable and relevant
We provide a large and expanding range of knockouts in immortalized mammalian cell lines, such as HeLa and HEK293T. All knockout cell lines are Sanger sequenced, and many have additional western blot data to confirm knockout at the proteomic level.
Ready to go
Ready-made cell lines mean you don’t need to spend months generating a KO cell line to get the results you need.
With our large range of ready-made knockouts in commonly used cell lines and our ever-expanding range of new targets and cell lines, obtaining your KO cell line of choice is as easy as buying a reagent.
Don’t see the cell line you need on our catalog? Register your interest for your desired knockout cell line and we will be in touch if it's in our pipeline. Click here to register your interest and be kept informed.
Frequently asked questions
??Get the answers to frequently asked questions about our knockout cell lines range.
- How are your Knockout (KO) cell lines generated?
- Do you test the KO cell lines for mycoplasma?
- Can I obtain a copy of the mycoplasma-free certificate?
- How should I store the cells once I receive them?
- Can I use my own WT cell line, or do I have to purchase it from you?
- Which wild type (WT) cell line should I purchase to use as a control with my KO cell line?
- What is the biological safety level (BSL) of your cells?
- What guarantees are in place for your KO cell lines?
- What is the passage number of the cell lines?
- Are there antibiotics in the cells?
- How should I culture my cells?
- What is the standard media formulation that your cells are in?
- Can't find your question? Contact us.
How are your Knockout (KO) cell lines generated?
Our KO cell lines were generated using CRISPR/Cas9 technology. The KO cell lines are single-clonal and validated using Sanger sequencing. Please refer to datasheet for the specific mutation description.
Do you test the KO cell lines for mycoplasma?
Yes, the cell line banks are tested for mycoplasma using an independent third party, Bionique, using broth and agar method. We also spot check our stocks regularly.
Can I obtain a copy of the mycoplasma-free certificate?
Yes. Please contact our scientific support team and they can provide this to you.
How should I store the cells once I receive them?
If you do not plan to resuscitate immediately, we recommend that you store the vial in liquid nitrogen immediately upon receipt.
Can I use my own WT cell line, or do I have to purchase it from you?
You can purchase the appropriate WT from us or receive it free of charge if ordered with a KO cell line, as these have been made by Abcam’s standard procedures. Please refer to the datasheet to determine which WT to order with your KO cell line. Alternatively, you can contact us if you have any questions.
Which wild type (WT) cell line should I purchase to use as a control with my KO cell line?
When you buy a KO cell line the WT is available free of charge, however you will need to add it separately to your order. Please refer to the KO cell line's datasheet for our recommended WT and how to order. If you have questions you can contact our scientific support team.
What is the biological safety level (BSL) of your cells?
Our KO cell lines are classified as BSL1 and BSL2 depending on the cell line. Cell lines such as A549, HCT116, HEPG2, and MCF7 are BSL1. EK-293T and HeLa cell lines are BSL2.
What guarantees are in place for your KO cell lines?
Our KO cell lines are covered by our Abpromise? guarantee. This means we guarantee the cell lines are mycoplasma free and validated at the genomic level by Sanger sequencing. ?
What is the passage number of the cell lines?
The cells are all less than passage number 20. Please contact us if you would like the exact passage number of your lot.
Are there antibiotics in the cells?
The clones are selected using puromycin resistance, however the cells are not grown in the presence of puromycin or other antibiotics.
How should I culture my cells?
Please refer to the handling procedure on the KO cell line's datasheet and our cell culture protocol here.
What is the standard media formulation that your cells are in?
The cells are in Cell Freezing Medium-DMSO Serum free media. Please refer to datasheet for more information.